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Metabolic Modelling as a Framework for Metabolomics Data Integration and Analysis
Open AccessArticle

Metabolic Profiling of a Porcine Combat Trauma-Injury Model Using NMR and Multi-Mode LC-MS Metabolomics—A Preliminary Study

Department of Chemistry, Faculty of Science, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore
Defence Medical and Environmental Research Institute, DSO National Laboratories, 27 Medical Drive, Singapore 117510, Singapore
Forensic Science Division, Health Services Authority, 11 Outram Road, Singapore 169078, Singapore
School of Applied Sciences, Temasek Polytechnic, 21 Tampines Ave 1, Singapore 529757, Singapore
Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore, Blk MD3, 16 Medical Drive, Singapore 117600, Singapore
NUS Environmental Research Institute, National University of Singapore, T-Lab Building, 5A Engineering Drive 1, Singapore 117411, Singapore
Authors to whom correspondence should be addressed.
Metabolites 2020, 10(9), 373;
Received: 30 May 2020 / Revised: 8 September 2020 / Accepted: 10 September 2020 / Published: 16 September 2020
(This article belongs to the Special Issue Animal and Cellular Models in Metabolomics Research)
Profiles of combat injuries worldwide have shown that penetrating trauma is one of the most common injuries sustained during battle. This is usually accompanied by severe bleeding or hemorrhage. If the soldier does not bleed to death, he may eventually succumb to complications arising from trauma hemorrhagic shock (THS). THS occurs when there is a deficiency of oxygen reaching the organs due to excessive blood loss. It can trigger massive metabolic derangements and an overwhelming inflammatory response, which can subsequently lead to the failure of organs and possibly death. A better understanding of the acute metabolic changes occurring after THS can help in the development of interventional strategies, as well as lead to the identification of potential biomarkers for rapid diagnosis of hemorrhagic shock and organ failure. In this preliminary study, a metabolomic approach using the complementary platforms of nuclear magnetic resonance (NMR) spectroscopy and liquid chromatography coupled with mass spectrometry (LC-MS) was used to determine the metabolic changes occurring in a porcine model of combat trauma injury comprising of penetrating trauma to a limb with hemorrhagic shock. Several metabolites associated with the acute-phase reaction, inflammation, energy depletion, oxidative stress, and possible renal dysfunction were identified to be significantly changed after a thirty-minute shock period. View Full-Text
Keywords: trauma; hemorrhagic shock; metabolomics; LC-MS; NMR trauma; hemorrhagic shock; metabolomics; LC-MS; NMR
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Laserna, A.K.C.; Lai, Y.; Fang, G.; Ganapathy, R.; Atan, M.S.B.M.; Lu, J.; Wu, J.; Uttamchandani, M.; Moochhala, S.M.; Li, S.F.Y. Metabolic Profiling of a Porcine Combat Trauma-Injury Model Using NMR and Multi-Mode LC-MS Metabolomics—A Preliminary Study. Metabolites 2020, 10, 373.

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