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Exercise- and Cold-Induced Human PGC-1α mRNA Isoform Specific Responses

School of Health and Kinesiology, University of Nebraska at Omaha, Omaha, NE 68182, USA
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Int. J. Environ. Res. Public Health 2020, 17(16), 5740; https://doi.org/10.3390/ijerph17165740
Received: 18 July 2020 / Revised: 4 August 2020 / Accepted: 7 August 2020 / Published: 8 August 2020
(This article belongs to the Section Exercise and Health)
Cold exposure in conjunction with aerobic exercise stimulates gene expression of PGC-1α, the master regulator of mitochondrial biogenesis. PGC-1α can be expressed as multiple isoforms due to alternative splicing mechanisms. Among these isoforms is NT-PGC-1α, which produces a truncated form of the PGC-1α protein, as well as isoforms derived from the first exon of the transcript, PGC-1α-a, PGC-1α-b, and PGC-1α-c. Relatively little is known about the individual responses of these isoforms to exercise and environmental temperature. Therefore, we determined the expression of PGC-1α isoforms following an acute bout of cycling in cold (C) and room temperature (RT) conditions. Nine male participants cycled for 1h at 65% Wmax at −2 °C and 20 °C. A muscle biopsy was taken from the vastus lateralis before and 3h post-exercise. RT-qPCR was used to analyze gene expression of PGC-1α isoforms. Gene expression of all PGC-1α isoforms increased due to the exercise intervention (p < 0.05). Exercise and cold exposure induced a greater increase in gene expression for total PGC-1α (p = 0.028) and its truncated isoform, NT-PGC-1α (p = 0.034), but there was no temperature-dependent response in the other PGC-1α isoforms measured. It appears that NT-PGC-1α may have a significant contribution to the reported alterations in the exercise- and temperature-induced PGC-1α response. View Full-Text
Keywords: RT-qPCR; transcription; truncated; alternate promoter; primary promoter RT-qPCR; transcription; truncated; alternate promoter; primary promoter
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Larson, C.; Opichka, M.; McGlynn, M.L.; Collins, C.W.; Slivka, D. Exercise- and Cold-Induced Human PGC-1α mRNA Isoform Specific Responses. Int. J. Environ. Res. Public Health 2020, 17, 5740.

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